Protein Crystallography
 
 
Protein Crystallography
 

We are thrilled with the Rigaku X-ray generator and Saturn CCD detector combination in our X-ray Core Facility at the University of Puerto Rico.

Recently, we were able to collect 1.09 Å data on our Rigaku system that allowed us to see the conformational changes that take place in blackfin tuna myoglobin upon S-nitrosylation (Journal of Biological Chemistry, 282 (2007) 19773).

This post-translational protein modification is extremely sensitive to X-ray radiation, so we were not able to collect synchrotron data and had to use our home source. The sensitivity of the Saturn CCD detector allowed us to minimize the exposure times and still collect atomic resolution data that was necessary to deconvolute multiple conformations of the S-nitrosylated cysteine.

—Dr. Eric R. Schreiter
Director, X-ray Crystallography Core Facility
Protein Research and Development Center
University of Puerto Rico

 
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Structure of the month

TetR/[Co Tc]+ complex from the laboratory of Dr. Winfried Hinrichs at the University of Greifswald
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Protein crystallography

In medicine, biotechnology and pharmacology, pharmaceutical discovery is the process by which therapeutic agents are identified or designed. Even in the recent past, most drugs have been discovered either by identifying the active ingredient in traditional remedies or by serendipitous discovery. A new approach, using so-called "rational drug design," has been to understand how disease and infection are controlled at the molecular level within proteins and other biological macromolecules so as to target specific entities based on this knowledge. A prerequisite for this approach is the ability to obtain high resolution three dimensional (3D) molecular structures of the protein, or other biologically active molecules, under investigation. Given the importance of this research, Rigaku has been at the forefront in the development of a wide range of instrumentation related to various aspects of protein crystallography.

Rigaku products range from automated crystallization and crystal imaging, through high-throughput crystal diffraction screening and high-resolution data collection systems, to 3D structure refinement and imaging software. A pioneer and world leader in designing and manufacturing X-ray measurement tools to solve single crystal X-ray diffraction challenges, Rigaku delivers cutting edge solutions for protein crystallography applications.

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