Life Science Product Areas
 
 
Protein Crystallography
Crystallization
Generators
 

We are thrilled with the Rigaku X-ray generator and Saturn CCD detector combination in our X-ray Core Facility at the University of Puerto Rico.

Recently, we were able to collect 1.09 Å data on our Rigaku system that allowed us to see the conformational changes that take place in blackfin tuna myoglobin upon S-nitrosylation (Journal of Biological Chemistry, 282 (2007) 19773).

This post-translational protein modification is extremely sensitive to X-ray radiation, so we were not able to collect synchrotron data and had to use our home source. The sensitivity of the Saturn CCD detector allowed us to minimize the exposure times and still collect atomic resolution data that was necessary to deconvolute multiple conformations of the S-nitrosylated cysteine.

—Dr. Eric R. Schreiter
Director, X-ray Crystallography Core Facility
Protein Research and Development Center
University of Puerto Rico

 
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Training Webinar
Structure Solution and Refinement with Phenix
Thursday, August 26, 2010
1:00 PM - 2 PM EDT



Presenter: Paul Adams

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Structure of the month

The structural charac­terization of the redundant thioredoxin system of Sulfolobus solfataricus

Product in the spotlight

 

Protein crystallography

Protein crystallography is a powerful structural biology technique that provides atomic resolution details about proteins and other macromolecular molecules involved in all aspects of life and disease. Rigaku is a world leader in providing instrumentation that can improve productivity at many levels—from making crystallization solutions and growing crystals to automatically mounting crystals and collecting high resolution X-ray diffraction data sets. Please select an area of interest below to find out how Rigaku can assist you in your protein crystallography research.

Complete X-ray systems
Equip your home laboratory with a completely integrated X-ray diffraction system for optimal performance including generator, optic, detector, software and crystal cryo-cooling. 

Complete X-ray systems

X-ray generators
Do more diffraction experiments in your home laboratory. Microfocus generators will provide the most intense X-ray sources ideal for protein crystallo­graphy—from high performance sealed tube systems to rotating anode generators that exceed the intensity of some second generation beamlines.

Rigaku generators

X-ray optics
Get the most out of your X-ray generator. High performance beam conditioning optics provide the best quality X-ray beam for protein crystal diffraction—high flux, excellent spectral purity, small beam size, and user adjustable divergence.

X-ray optics

Area detectors 
Accurately collect the best quality diffraction data with two-dimensional area detectors based on either imaging plate (IP) or CCD technology. IP detectors are the general purpose workhorses in home X-ray labs while CCD detectors offer faster read out times and are ideal for groups that require higher throughput. Both types of detectors enable you to collect high quality diffraction data in your home lab.

Area detectors

Automated protein crystal handling
Increase the number and quality of your diffraction experiments by screening more crystals and collecting data sets automatically, even at night and over the weekend. A reliable robotic system will automatically mount your samples, center them in the X-ray beam and then return them to a liquid nitrogen filled dewar when screening or data collection is complete.

Automated protein crystal handling

 

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