Protein Crystallography

We are thrilled with the Rigaku X-ray generator and Saturn CCD detector combination in our X-ray Core Facility at the University of Puerto Rico.

Recently, we were able to collect 1.09 Å data on our Rigaku system that allowed us to see the conformational changes that take place in blackfin tuna myoglobin upon S-nitrosylation (Journal of Biological Chemistry, 282 (2007) 19773).

This post-translational protein modification is extremely sensitive to X-ray radiation, so we were not able to collect synchrotron data and had to use our home source. The sensitivity of the Saturn CCD detector allowed us to minimize the exposure times and still collect atomic resolution data that was necessary to deconvolute multiple conformations of the S-nitrosylated cysteine.

—Dr. Eric R. Schreiter
Director, X-ray Crystallography Core Facility
Protein Research and Development Center
University of Puerto Rico

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The structural characterization of the redundant thioredoxin system of Sulfolobus solfataricus

Macromolecular Crystallography Webinar training series

Welcome to the Rigaku Life Sciences Webinar Series. This free webinar series is designed to provide educational and training materials in X-ray crystallography methods. Each webinar will address a new topic ranging from optimization of crystallization conditions to solving the phase problem. We invite anyone to submit topic suggestions for future webinars and hope that you'll provide feedback for previous webinars.

Next webinar:

Title: Structure Solution and Refinement with Phenix
Presenter: Paul Adams
Date: Thursday, August 26, 2010
Time: noon - 1:00 PM CDT (1:00 PM - 2:00 PM EDT / 17:00 - 18:00 GMT)

Paul Adams

Phenix is a Python-based software system for the solution and refinement of crystallographic structures using X-ray and/or neutron diffraction data. Recent developments in Phenix, especially in the refinement program phenix.refine, are presented. These include real space target functions for refinement, the use of torsion angle constraints, and the application of additional restraints to improve structure refinement at low resolution. The integration of automated side chain rotamer correction into the refinement process will also be described.

Reference: Adams P.D., Afonine P.V., Bunkóczi G., Chen V.B., Davis I.W., Echols N., Headd J.J., Hung L.-W., Kapral G.J., Grosse-Kunstleve R.W., McCoy A.J., Moriarty N.W., Oeffner R., Read R.J., Richardson D.C., Richardson J.S., Terwilliger T.C., Zwart P.H., Acta Cryst. 2010, D66, 213.

To register for the webinar, please fill out this form.

Past webinars:

To view and/or download past webinars click here.

To find information about future webinars, check this web site frequently. Upcoming webinars are also included in our monthly Life Sciences newsletter. If you would like to receive this monthly newsletter send email to info@rigaku.com.

Other training courses:

Rigaku Americas is pleased to offer valuable training sessions to teach you and your staff how to keep your Rigaku diffraction equipment running in excellent shape and how to extend the lifetime of your equipment by hands-on training from service specialists. Learn more.

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